Optimized microbial DNA extraction from diarrheic stools

An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments

In diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. If not inactivated properly, cellular and/or environmental DNA nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in PCR experiments. When crude cell extracts, proteinase K–treat...

Comparative detection and quantification of Arcobacter butzleri in stools from diarrheic and non-diarrheic human beings in southwestern Alberta, Canada

Seasonal variation on the presence of adenoviruses in stools from non-diarrheic patients

Human adenoviruses (HAdV), members of the Adenoviridae family, are excreted through the fecal route and may be present in the feces of humans consuming contaminated food or water. The presence of HAdV from different serotypes in the feces of healthy individuals was already reported using conventional polymerase chain reaction; however, real-time PCR (qPCR) may reveal not only the rates of detec...

Extraction of high molecular weight DNA from microbial mats.

Due to the presence of inhibitors such as extracellular polymeric substances (EPSs) and salts, most microbial mat studies have relied on harsh methods of direct DNA extraction that result in DNA fragments too small for large-insert vector cloning. High molecular weight (HMW) DNA is crucial in functional metagenomic studies, because large fragments present greater access to genes of interest. He...

The extraction and purification of microbial DNA from sediments

A new method for the isolation of intracellular and extracellular DNA from a range of sediment types has been developed. This method is based upon the direct lysis of cells in the sediment, extraction of released DNA from the sediments and its subsequent purification by CsC1-EtBr gradient centrifugation and/or hydroxyapatite chromatography. Yields of 26 ~g intracellular DNA and 1 #g extracellul...